Search results for "Animal cell culture"

showing 3 items of 3 documents

Mass transfer and hydrodynamic characteristics of unbaffled stirred bio-reactors: Influence of impeller design

2014

Abstract Unbaffled stirred tanks are increasingly recognized as a viable alternative to common baffled tanks for a range of processes where the presence of baffles is undesirable for some reason. For instance, in the case of shear sensitive cell cultivation (e.g. human cells), unbaffled tanks have been recently found to be able to provide sufficient mass transfer through the free surface vortex. As a consequence the need for bubble formation and subsequent bursting, along with relevant cells damage, is conveniently avoided. In this work the influence of impeller geometry on mass transfer performance and power demand of an unbaffled stirred vessel operating both in sub-critical conditions (t…

Mass transfer coefficientEngineeringEnvironmental EngineeringMass transfer coefficientbusiness.industrySettore ING-IND/25 - Impianti ChimiciMIXINGBiomedical EngineeringMixing (process engineering)Mechanical engineeringBioengineeringBaffleMechanicsMultiphase bioreactorVortexMixing Multiphase reactors Unbaffled tanks Mass transfer coefficient kLa Bioreactors Animal cell cultureImpellerFree surfaceMass transferLiquid bubblebusinessBiotechnologyAnimal cell culture
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Unbaffled, Stirred Bioreactors for Animal Cell Cultivation

2017

One of the main features of animal cell bioreactors is that the cultured cells lack a strong membrane and are therefore more prone to shear damage. It is widely accepted that animal cell damage in aerated bioreactors is mainly related to burst bubbles at the air–liquid interface. A viable alternative to sparged bioreactors, aimed at minimizing cell damage, may be represented by uncovered, unbaffled, stirred tanks, which are able to provide sufficient mass transfer through the deep free surface vortex that takes place under agitation. As a consequence the need for bubble formation and subsequent bursting accompanied by cell damage is conveniently avoided. In this chapter, mass transfer perfo…

Mass transfer coefficientMass transfer coefficientMaterials scienceSettore ING-IND/25 - Impianti ChimiciMixing timeBioreactortechnology industry and agricultureMixing (process engineering)Mechanical engineeringMechanicsequipment and suppliesmedicine.diseaseImpellerkLaUnbaffled tanksMixingFree surfaceMass transferMultiphase reactorBioreactormedicineFree surface shapeLiquid bubbleCell damageAnimal cell culture
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Smad7 controls resistance of colitogenic T cells to regulatory T cell-mediated suppression.

2008

Background & Aims Foxp3-expressing regulatory T cells (Tregs) play a key role in the maintenance of the gut immune homeostasis, and an intact transforming growth factor (TGF)-β signaling is required for their function. In inflammatory bowel disease (IBD), the TGF-β signaling is impaired because of high expression of the inhibitory molecule Smad7. Although no intrinsic defects in Tregs function have been shown in IBD, it is still unknown whether colitogenic T cells are susceptible to Treg-mediated suppression. In this study, we have investigated whether IBD mucosal CD4+ T cells are resistant to Tregs and whether Smad7 is involved in this process. Methods IBD lamina propria mononuclear cells …

antisense oligonucleotideCD4-Positive T-LymphocytesAdoptive cell transferT-Lymphocytesanimal cellCell CommunicationInbred C57BLT-Lymphocytes RegulatoryTransgenicMiceregulatory T lymphocyteCrohn DiseaseTransforming Growth Factor betamononuclear cellRAG1 proteinIntestinal MucosaenteritisCells CulturedMice KnockoutSettore MED/12 - GastroenterologiaCulturedintegumentary systemmedicine.diagnostic_testarticleGastroenterologyInterleukinhemic and immune systemsT helper cellColitisRegulatoryUp-Regulationmedicine.anatomical_structurepriority journalgamma interferonSignal TransductionRegulatory T cellColonCellsKnockoutanimal experimentinterleukin 6chemical and pharmacologic phenomenaMice TransgenicBiologyinterleukin 2Recombination-activating geneFlow cytometryProinflammatory cytokineSmad7 ProteinmedicineAnimalsHumanscontrolled studyhumanlamina propriamouseCell ProliferationHomeodomain ProteinsCD4+ T lymphocytenonhumanHepatologyAnimalflow cytometryhuman cellanimal cell culturetransgenic mouseMice Inbred C57BLDisease Models Animalantisense oligonucleotide; gamma interferon; interleukin 17; interleukin 2; interleukin 6; RAG1 protein; Smad7 protein; animal cell; animal cell culture; animal experiment; article; CD4+ T lymphocyte; cell proliferation; colitis; controlled study; enteritis; flow cytometry; human; human cell; knockout mouse; lamina propria; mononuclear cell; mouse; nonhuman; priority journal; regulatory T lymphocyte; transgenic mouse; Animals; CD4-Positive T-Lymphocytes; Cell Communication; Cell Proliferation; Cells Cultured; Colitis; Colon; Crohn Disease; Disease Models Animal; Homeodomain Proteins; Humans; Intestinal Mucosa; Mice; Mice Inbred C57BL; Mice Knockout; Mice Transgenic; Signal Transduction; Smad7 Protein; T-Lymphocytes Regulatory; Transforming Growth Factor beta; Up-RegulationDisease ModelsImmunologyinterleukin 17knockout mouseTransforming growth factorGastroenterology
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